Rapid light-induced surface charge changes in bacteriorhodopsin
نویسندگان
چکیده
منابع مشابه
Electric-field induced pK-changes in bacteriorhodopsin.
Bacteriorhodopsin, the only protein of the purple membranes of halobacteria [ 11, acts as a light-driven proton pump, translocating protons from the inner to the outer side of the plasma membrane [2]. The lightinduced asymmetric proton distribution contributes to the membrane potential by up to lo-40 mV [3]. Assuming a membrane thickness of 50 A, this potential difference corresponds to an elec...
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Electric impulses of high field intensity (2 x 105 to 3 × 106 Vm1, 1 to 20 #s duration) cause transient changes in the optical absorbance of suspended purple membranes ofHalobacterium halobium. The electric dichroism at 1 mM NaCl, pH ~ 6,and at 293K is dependent on field strength, pulse duration and wavelength of the monitoring, plane-polarized light in the range 400 to 650 nm. The optically de...
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We have recently introduced a method, made possible by an improved orienting technique using a combination of electric and magnetic fields, that allows the three-dimensional detection of the intramolecular charge displacements during the photocycle of bacteriorhodopsin. This method generates electric asymmetry, a prerequisite for the detection of electric signal on the macroscopic sample, in al...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1982
ISSN: 0014-5793
DOI: 10.1016/0014-5793(82)80022-7